4G7V

Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.5 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Detector	MAR 300 CCD
Spacegroup name	P 65 2 2
Unit cell lengths	120.250, 120.250, 229.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.238 - 2.500
R-factor	0.2028
Rwork	0.201
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	33756
Completeness [%]	99.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M SPG, 20% PEG 1500, 10% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M SPG, 20% PEG 1500, 10% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K