4G7H
Crystal structure of Thermus thermophilus transcription initiation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.560, 104.570, 297.550 |
| Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
| Resolution | 49.270 - 2.900 |
| R-factor | 0.1884 |
| Rwork | 0.188 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.101 | 0.667 |
| Number of reflections | 249337 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
