4G2K
Crystal structure of the Marburg Virus GP2 ectodomain in its post-fusion conformation
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.574, 147.529, 42.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ebo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.071 | 0.036 | 0.775 |
| Number of reflections | 26888 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.6 | 99.2 | 94.3 |
| Redundancy | 9.7 | 9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.17 M sodium acetate, 0.085 M Tris-HCl, pH 8.5, 25.5% PEG3350, 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
