4G0R

Structural characterization of H-1 Parvovirus: comparison of infectious virions to replication defective particles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	277
Detector technology	CCD
Collection date	2010-06-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9186
Spacegroup name	P 1 21 1
Unit cell lengths	255.400, 350.400, 271.600
Unit cell angles	90.00, 90.34, 90.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.216
R-free	0.21690
RMSD bond length	0.006
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	1127217
<I/σ(I)>	6.8	2.4
Completeness [%]	93.1	94.7
Redundancy	2.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	The reservoir solution contained 1-3% (w/v) polyethylene glycol 8000 (PEG 8000), 150 mM NaCl, and 8mM CaCl2.2H2O as precipitants in 10 mM Tris. The drops were prepared by mixing equivolume of virus solution (10mg/ml) in Tris-HCl Buffer (10 mM Tris-HCl, 150 mM NaCl, 8mM CaCl2.2H2O) with reservoir solution, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K