4G0N
Crystal Structure of wt H-Ras-GppNHp bound to the RBD of Raf Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 90.440, 90.440, 92.703 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.368 - 2.450 |
R-factor | 0.1858 |
Rwork | 0.181 |
R-free | 0.22940 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.680 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.400 |
High resolution limit [Å] | 2.450 |
Rmerge | 0.150 |
Number of reflections | 16218 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | Protein solution: 10 - 16 mg/mL, 50 mM HEPES, pH 7.2, 50 mM NaCl, 10mM MgCl2 5% Glycerol, 1mM DTE, 10 M ZnCl2 Reservoir solution:200mM calcium acetate, 100mM sodium cacodylate pH 6.5, 18% PEG 8000. Drop: 1uL protein, 1uL reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 291K |