4G01
ARA7-GDP-Ca2+/VPS9a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.349, 76.139, 81.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.730 - 2.200 |
| R-factor | 0.20173 |
| Rwork | 0.198 |
| R-free | 0.27282 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2efc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 22345 | |
| <I/σ(I)> | 15.8 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 200mM calcium acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
