4FYD

Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA and dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	111.689, 145.686, 197.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.801 - 3.100
R-factor	0.2116
Rwork	0.210
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fvm
RMSD bond length	0.003
RMSD bond angle	0.652
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1078))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	3.260
High resolution limit [Å]	3.100	3.100
Rmerge	0.105	0.974
Number of reflections	59154
<I/σ(I)>	10.8	2.1
Completeness [%]	99.7	98.4
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	0.2M MgAc2, 10% PEG8000, microbatch, temperature 291K