4FXW
Structure of phosphorylated SF1 complex with U2AF65-UHM domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9219, 0.9794, 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.800, 116.100, 130.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.560 - 2.290 |
| R-factor | 0.2513 |
| Rwork | 0.248 |
| R-free | 0.28070 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.290 | 6.100 |
| Rmerge | 0.062 | 0.039 |
| Number of reflections | 50161 | |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 99.6 | 95.5 |
| Redundancy | 4.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M Lithium sulfate, 20% PEG 3350 and 0.1 M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
