4FQC
Crystal Structure of PGT121 Fab Bound to a complex-type sialylated N-glycan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-09 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.787, 67.787, 94.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.656 - 2.400 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.949 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.656 | 2.330 |
High resolution limit [Å] | 2.209 | 2.210 |
Rmerge | 0.691 | |
Number of reflections | 20621 | |
<I/σ(I)> | 8.7 | 1.1 |
Completeness [%] | 92.2 | 65.4 |
Redundancy | 5.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 298 | PEG 10000, Bis Tris HCl, CH3COOHNH4, pH 5.5, vapor diffusion, temperature 298K |