4FP8
Crystal structure of broadly neutralizing antibody C05 bound to H3 influenza hemagglutinin, HA1 subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.794, 247.720, 95.380 |
Unit cell angles | 90.00, 91.33, 90.00 |
Refinement procedure
Resolution | 44.495 - 2.947 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4FNK AND 4FNL |
RMSD bond length | 0.005 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.921 | 2.921 |
Number of reflections | 84287 | |
<I/σ(I)> | 10.9 | 2.1 |
Completeness [%] | 95.5 | 97.2 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 10% w/v PEG3000, 200 mM zinc acetate, 100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |