4FLY
Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.991870 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.100, 114.340, 128.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.000 - 2.300 |
R-factor | 0.1778 |
Rwork | 0.176 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4flw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.320 |
Rmerge | 0.141 | 0.660 |
Number of reflections | 44918 | |
<I/σ(I)> | 10.7 | 2.6 |
Completeness [%] | 97.8 | 97.3 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-12% PEG 20000, 100 mM MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |