4FKK

Crystal structure of porcine aminopeptidase-N complexed with bestatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	260.611, 63.014, 81.705
Unit cell angles	90.00, 100.83, 90.00

Refinement procedure

Resolution	28.280 - 2.600
R-factor	0.16974
Rwork	0.164
R-free	0.27562
Structure solution method	MIRAD
RMSD bond length	0.010
RMSD bond angle	1.471
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	39425
Completeness [%]	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	18% PEG3350, 200 mM lithium sulfate, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	18% PEG3350, 200 mM lithium sulfate, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K