4FKB
An Organic solvent tolerant lipase 42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-02-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.824, 81.001, 99.529 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.220 |
| R-factor | 0.12894 |
| Rwork | 0.127 |
| R-free | 0.16458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.252 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.056 | |
| Number of reflections | 275120 | |
| Completeness [%] | 93.7 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 293.15 | Counter-diffusion method without mixing the protein and precipitant, pH 8, LIQUID DIFFUSION, temperature 293.15K |
