4FIF
Catalytic domain of human PAK4 with RPKPLVDP peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 3 |
| Unit cell lengths | 141.411, 141.411, 61.624 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.460 - 2.600 |
| R-factor | 0.20684 |
| Rwork | 0.206 |
| R-free | 0.22735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cdz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.887 |
| <I/σ(I)> | 17.5 | 1.8 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M MES, 700 mM K/Na tartrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
