4FAI
Crystal structure of mitochondrial isoform of glutaminyl cyclase from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.443, 47.734, 74.556 |
| Unit cell angles | 85.03, 74.89, 73.90 |
Refinement procedure
| Resolution | 71.969 - 1.650 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f9v |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.969 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.489 |
| Number of reflections | 69017 | |
| <I/σ(I)> | 14.1 | 2.3 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 30% PEG4000, 0.2 M magnesium chloride, 2 mM PQ50, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
