4F8C
Structure of the Cif:Nedd8 complex - Yersinia pseudotuberculosis Cycle Inhibiting Factor in complex with human Nedd8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00720 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 125.359, 125.359, 169.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.810 - 1.950 |
| R-factor | 0.18923 |
| Rwork | 0.187 |
| R-free | 0.23479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fbj |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.900 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 57931 | |
| <I/σ(I)> | 18.5 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2.2M sodium malonate, 44mM bis-tris propane (pH 7.0), 66mM bis-tris propane (pH 8.0), VAPOR DIFFUSION, SITTING DROP, temperature 289K |
