4F7U
The 6S snRNP assembly intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.720, 65.298, 99.346 |
| Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
| Resolution | 49.627 - 1.898 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4F77 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 88058 | |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 2.69 | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% Jeffamine ED-2003 titrated to pH 7, 10% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
