4F7G

Crystal structure of talin autoinhibition complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979150
Spacegroup name	P 21 21 21
Unit cell lengths	36.665, 86.876, 113.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.020 - 2.050
R-factor	0.21245
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.020	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	20930
Completeness [%]	89.2	51.2
Redundancy	8.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	18.5% (w/v) PEG 1500, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
2	VAPOR DIFFUSION, HANGING DROP	6.5	289	18.5% (w/v) PEG 1500, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K