4F5T
Crystal Structure of Equine Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 61 |
| Unit cell lengths | 96.573, 96.573, 143.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.320 |
| R-factor | 0.19913 |
| Rwork | 0.197 |
| R-free | 0.25852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f5s |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.087 | 0.521 |
| Number of reflections | 31502 | |
| <I/σ(I)> | 17.4 | 1.93 |
| Completeness [%] | 94.6 | 68.7 |
| Redundancy | 6.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.7 M ammonium sulfate, 0.1M acetate buffer pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
