4F2T

Modulation of S.aureus Phosphatidylinositol-Specific Phospholipase C Membrane Binding.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2012-01-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	86.088, 55.957, 61.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.855 - 2.300
R-factor	0.1898
Rwork	0.187
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v18
RMSD bond length	0.008
RMSD bond angle	1.086
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((phaser))
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.063
Completeness [%]	98.9	97.8
Redundancy	12.3	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	16% PEG 4000, 0.150 M ammonium acetate, 0.100 M sodium acetate, 0.100 M magnesium nitrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K