4F2D

Crystal Structure of Escherichia coli L-arabinose Isomerase (ECAI) complexed with Ribitol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	116.472, 116.472, 214.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.21401
Rwork	0.212
R-free	0.25505
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ajt
RMSD bond length	0.006
RMSD bond angle	1.077
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	85584
<I/σ(I)>	22.3
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, TRISODIUM CITRATE DIHYDRATE, Ribitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, TRISODIUM CITRATE DIHYDRATE, Ribitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K