4F23
Influenza A virus hemagglutinin H16 HA0 structure with an alpha-helix conformation in the cleavage site: a potential drug target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Collection date | 2012-05-03 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.332, 240.944, 69.673 |
Unit cell angles | 90.00, 119.79, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.700 |
R-factor | 0.16143 |
Rwork | 0.160 |
R-free | 0.19222 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 1.904 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.500 | 1.770 |
High resolution limit [Å] | 1.700 | 1.710 |
Number of reflections | 200026 | |
Completeness [%] | 98.5 | 91.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Imidazole, 20% (v/v) Jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |