4F22
Kainate bound to the K660A mutant of the ligand binding domain of GluA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 47.657, 49.028, 136.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.150 - 2.060 |
| R-factor | 0.1845 |
| Rwork | 0.181 |
| R-free | 0.24362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dln |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.897 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.080 |
| Rmerge | 0.157 | 0.312 |
| <I/σ(I)> | 11.112 | 2.261 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 14-15% PEG 8K, 0.1 M sodium cacodylate, 0.1-0.15 M zinc acetate, 0.25 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
