4F1I
Crystal structure of SeMet TDP2 from Caenorhabditis elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 96 |
Detector technology | CCD |
Collection date | 2011-09-09 |
Detector | NOIR-1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 100.685, 100.685, 121.375 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.185 - 2.500 |
R-factor | 0.1845 |
Rwork | 0.182 |
R-free | 0.22880 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.878 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.126 | 0.828 |
Number of reflections | 23342 | |
<I/σ(I)> | 8.3 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 293 | PEG 6.000, 0.3M ammonium chloride, and 0.1M MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K |