4F1E
The Crystal Structure of a Human MitoNEET mutant with Asp 67 replaced by a Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.396, 110.855, 207.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.540 - 2.400 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 2QH7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.540 | 2.490 | |
High resolution limit [Å] | 2.400 | 5.140 | 2.400 |
Rmerge | 0.069 | 0.049 | 0.754 |
Number of reflections | 51597 | 5584 | 5070 |
<I/σ(I)> | 15.34 | 33.51 | 1.87 |
Completeness [%] | 99.4 | 99.5 | 94.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |