4F0X
Crystal structure of human Malonyl-CoA Decarboxylase (Peroxisomal Isoform)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2011-06-16 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.420, 103.310, 134.240 |
| Unit cell angles | 95.32, 90.22, 94.46 |
Refinement procedure
| Resolution | 66.800 - 3.290 |
| R-factor | 0.2456 |
| Rwork | 0.244 |
| R-free | 0.26670 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.820 | 3.470 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Number of reflections | 63973 | |
| <I/σ(I)> | 4.9 | 2 |
| Completeness [%] | 97.7 | 97.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 16% PEG 2000 MME 0.1M AcNa 10mM CitNa, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
