4EZH

the crystal structure of KDM6B bound with H3K27me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-11
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	83.682, 102.413, 143.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.520
R-factor	0.215
Rwork	0.213
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.466
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.111	0.630
Number of reflections	41888
<I/σ(I)>		1.8
Completeness [%]	99.9	100
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2	293	11% methanol, 6% MPD, 5% PEG 4K, 5% Glycerol, HEPES 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K