4EZ6

Bacillus DNA Polymerase I Large Fragment Complex 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Collection date	2009-10-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97121
Spacegroup name	P 21 21 21
Unit cell lengths	93.740, 109.510, 149.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.870 - 1.640
R-factor	0.1717
Rwork	0.171
R-free	0.19220
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED
RMSD bond length	0.010
RMSD bond angle	1.355
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (dev_1026)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.740
High resolution limit [Å]	1.640	4.880	1.640
Rmerge	0.055	0.025	0.457
Number of reflections	166080
<I/σ(I)>	16.63	46.53	3.63
Completeness [%]	88.1	86.6	98.3
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	48%-53% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES (pH 5.8), VAPOR DIFFUSION, HANGING DROP, temperature 290K