4EWS
Crystal structure of cholesteryl ester transfer protein in complex with inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.600, 69.323, 188.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.590 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.25910 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2obd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 22976 |
| Completeness [%] | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10mg/ml protein 1:1 mixing with 0.1M HEPES, pH=7.5, 0.2 M MgCl2 and 27-35% (W/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
