4ES0
X-ray structure of WDR5-SETd1b Win motif peptide binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | CCD |
| Collection date | 2009-09-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.917 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.254, 98.272, 79.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.852 - 1.817 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.154 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 3.900 | 1.810 |
| Rmerge | 0.130 | 0.087 | 0.377 |
| Number of reflections | 27686 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 98.3 | 97 | 89.8 |
| Redundancy | 13.2 | 12.5 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.6 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.6, hanging drop, temperature 294K |
