4EP7
Functional implications from the Cid1 poly(U) polymerase crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9811 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.490, 76.860, 81.860 |
Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
Resolution | 45.193 - 2.280 |
R-factor | 0.1907 |
Rwork | 0.188 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.112 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_353)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.193 | 4.000 | 2.300 |
High resolution limit [Å] | 2.270 | 3.000 | 2.270 |
Rmerge | 0.130 | ||
Number of reflections | 29999 | ||
<I/σ(I)> | 11.5 | 15.88 | 2.64 |
Completeness [%] | 97.4 | 99.2 | 59.3 |
Redundancy | 2.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 291 | 0.1M Imidatole/MES pH 6.1, 20% Glycerol, 10% PEG 4000, 42mM NaI, 42mM NaBr, 42mM NaF., VAPOR DIFFUSION, SITTING DROP, temperature 291K |