4EP5
Thermus thermophilus RuvC structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 35.183, 60.019, 135.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.130 - 2.080 |
| R-factor | 0.213 |
| Rwork | 0.208 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.974 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1005)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.070 | 2.080 |
| Rmerge | 0.060 | 0.698 |
| Number of reflections | 8463 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 93.3 | 0.7 |
| Redundancy | 6.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 293 | PEG400, Li2SO4, and sodium acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
