4EO7

Crystal structure of the TIR domain of human myeloid differentiation primary response protein 88.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	34.876, 56.810, 66.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.239 - 1.449
R-factor	0.17
Rwork	0.168
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dom
RMSD bond length	0.006
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.449	1.449
Number of reflections	23051
Completeness [%]	95.2	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	25% PEG 3350, 0.1M BIS-TRIS pH 6.5,0.2M NaCl, VAPOR DIFFUSION, temperature 277K