4EO4
Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase (MST1) in complex with seryl sulfamoyl adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 76 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.233, 107.498, 153.391 |
| Unit cell angles | 90.00, 130.05, 90.00 |
Refinement procedure
| Resolution | 49.722 - 2.870 |
| R-factor | 0.1777 |
| Rwork | 0.175 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Number of reflections | 60275 | |
| <I/σ(I)> | 15 | 2.2 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 285 | 0.1M di-sodium phosphate / mono-potassium phosphate, 0.2M NaCl, 10 % (w/v) PEG 8,000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
