4ENO

Crystal structure of oxidized human nm23-H1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 21 3
Unit cell lengths	106.768, 106.768, 106.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.748 - 2.800
R-factor	0.1895
Rwork	0.182
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.315
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.755
High resolution limit [Å]	2.800	2.800
Number of reflections	10255
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	296	2.0M sodium potassium phosphate pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K