4ENK
Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-propylguanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.501, 59.501, 236.917 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.879 - 3.045 |
R-factor | 0.2544 |
Rwork | 0.250 |
R-free | 0.29520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gva |
RMSD bond length | 0.010 |
RMSD bond angle | 1.651 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.045 | 3.045 |
Number of reflections | 5247 | |
Completeness [%] | 97.6 | 82.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 288 | 25% mPEG 2000, 5% sodium formate, 0.2M imidazole-malate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |