4EMO
Crystal structure of the PH domain of SHARPIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | ADSC |
| Wavelength(s) | 0.97990 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.550, 61.550, 222.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2122 |
| Rwork | 0.209 |
| R-free | 0.26910 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.135 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 4.000 | 2.000 |
| Rmerge | 0.071 | 0.042 | 0.483 |
| Number of reflections | 29888 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 99.1 | 96.6 | 98.3 |
| Redundancy | 3.9 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 4M sodium formate, pH 7.4, vapor diffusion, sitting drop, temperature 291K |
