4EMA
Human peroxisome proliferator-activated receptor gamma in complex with rosiglitazone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.46 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.511, 61.958, 117.601 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 28.890 - 2.545 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.890 | 2.640 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 20835 | |
| Completeness [%] | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M tris-HCl, 0.9M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
