4ELL
Structure of the inactive retinoblastoma protein pocket domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0971 |
| Spacegroup name | H 3 |
| Unit cell lengths | 249.659, 249.659, 35.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.035 - 1.980 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.180 | 2.080 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 57109 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 100mM sodium citrate, 1mM LiCl, 18% PEG 8K., pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
