4EKY
Crystal structure of GPb in complex with DK15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.674, 128.674, 116.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.570 - 2.450 |
| R-factor | 0.16435 |
| Rwork | 0.161 |
| R-free | 0.23038 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3sym |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.771 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.700 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 33602 | |
| <I/σ(I)> | 13.8 | 2.3 |
| Completeness [%] | 82.4 | |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10mM BES buffer, pH 6.7, SMALL TUBES, temperature 289K |
