4EJ7

Crystal structure of the aminoglycoside phosphotransferase APH(3')-Ia, ATP-bound

Replaces:  3R78

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97940
Spacegroup name	C 2 2 21
Unit cell lengths	85.363, 152.466, 165.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.669 - 2.290
R-factor	0.215
Rwork	0.213
R-free	0.25160
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.694
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.669	2.300
High resolution limit [Å]	2.290	2.290
Number of reflections	91198
<I/σ(I)>	12.552	1.5
Completeness [%]	91.9	69.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M CA ACETATE, 16% PEG3350, 2 MM ATP, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K