4EIK
Crystal Structure of the Human Fyn SH3 domain in complex with the synthetic peptide VSL12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.843, 81.843, 35.904 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.658 - 1.600 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.19540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ua7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.928 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.658 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.031 | 0.230 |
| Number of reflections | 17446 | |
| <I/σ(I)> | 71.1 | 10 |
| Completeness [%] | 94.4 | 72.5 |
| Redundancy | 11 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 5.5 M sodium formate, 0.1 M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
