4EHI
An X-ray Crystal Structure of a putative Bifunctional Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.881, 101.234, 114.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.480 - 2.280 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 2.300 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.095 | 0.549 |
| Number of reflections | 48301 | |
| <I/σ(I)> | 36.75 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20%PEG 3350, 0.2M Na Sulfate, 0.1M Bis-Tris, 5.8mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
