4EGC
Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2011-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.207, 150.180, 53.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.032 - 1.994 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.22410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 4.020 | 1.480 |
| Rmerge | 0.081 | 0.031 | |
| Number of reflections | 145786 | ||
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 87.6 | 99.7 | 39.8 |
| Redundancy | 7.1 | 9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 291 | 13.75% PEG8000, 0.01 M magnesium chloride, 0.05 M MES, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
