4EEY
Crystal structure of human DNA polymerase eta in ternary complex with a cisplatin DNA adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.500, 98.500, 82.603 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.250 - 2.320 |
| R-factor | 0.18247 |
| Rwork | 0.178 |
| R-free | 0.23253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mr2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.462 | |
| Number of reflections | 19734 | |
| <I/σ(I)> | 16.9 | 3.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15-35% PEG1500, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
