4EDN
Crystal structure of beta-parvin CH2 domain in complex with paxillin LD1 motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2011-07-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 330.741, 55.842, 95.550 |
| Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 38837 | |
| <I/σ(I)> | 19.829 | 2.132 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2.1M Ammonium sulfate, 0.01M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
