4EC8
Structure of full length CDK9 in complex with cyclinT and DRB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.976 |
| Spacegroup name | H 3 |
| Unit cell lengths | 175.050, 175.050, 101.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.871 - 3.600 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.690 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.711 | |
| Number of reflections | 13165 | |
| <I/σ(I)> | 1.4 | |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 1% PEG 1K, 180MM NAK PHOSOPHATE 4mM TCEP, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
