4EBK

Crystal structure of aminoglycoside 4'-O-adenylyltransferase ANT(4')-IIb, tobramycin-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-22
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	81.906, 84.451, 98.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.893 - 2.150
R-factor	0.1741
Rwork	0.172
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ebj
RMSD bond length	0.008
RMSD bond angle	1.142
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.190
High resolution limit [Å]	2.150	2.150
Number of reflections	37909
<I/σ(I)>	16.45	2.02
Completeness [%]	99.9	100
Redundancy	7.6	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M sodium iodide, 20% PEG 3350, 10 mM tobramycin, 5 mM ATP, cryoprotectant: 25% ethylene glycol, 2 mM CaCl2, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K