4EA3
Structure of the N/OFQ Opioid Receptor in Complex with a Peptide Mimetic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.106, 170.939, 65.353 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 32.019 - 3.013 |
| R-factor | 0.2505 |
| Rwork | 0.248 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4djh 1m6t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.563 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.190 | 0.660 |
| Number of reflections | 16545 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 93.3 | 79.5 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Lipidic Cubic Phase (LCP) | 6.4 | 293 | 25-30% (v/v) PEG 400, 100 to 200 mM potassium sodium tartrate tetrahydrate, 100 mM BIS-TRIS propane pH 6.4 , Lipidic Cubic Phase (LCP), temperature 293K |
