4E9D
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with 3-(1-benzothiophen-2-yl)propanoyl-derivatized DPPLHSpTA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Detector technology | CCD |
| Collection date | 2011-03-15 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.873, 36.302, 47.474 |
| Unit cell angles | 85.84, 77.21, 68.24 |
Refinement procedure
| Resolution | 46.290 - 2.750 |
| R-factor | 0.26984 |
| Rwork | 0.267 |
| R-free | 0.32984 |
| Structure solution method | 3P2Z |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.765 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.290 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 5422 |
| Completeness [%] | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate, 1.0 M LiCl, 30% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
